14,000-Year-Old Poop Found in Oregon Cave Turns Out to Be Human

Gizmodo reports that archaeologists John Blong and Lisa-Marie Shillito of Newcastle University and their colleagues tested 21 coprolites unearthed in Oregon’s Paisley Caves for the presence of human sterols and bile, which are not soluble in water and thus chemically stable. Previous mitochondrial DNA testing of the ancient waste indicated that all of the samples were human in origin, but critics argued that DNA from later occupation of the cave may have washed into lower, older cave sediments and contaminated the samples.

“We address issues of potential DNA contamination through fecal lipid biomarker analysis, providing evidence that there likely was DNA moving from younger human occupations into older cave sediments and coprolites, but also confirming that people were camping at the caves as early as 14,200 years ago,” Blong said.

Genetic analysis of the coprolites suggested they came from humans, but some researchers questioned this result, citing possible contamination of the samples. The progeny of the poop remained unresolved for years, but new research is providing a fresh look at these stale but incredibly important piles of dung.

Humans first entered North America around the end of the last ice age, sometime between 20,000 and 15,000 years ago. Further confirmation of exactly when and how this migration took place would be a big deal, even if the evidence in question is literally full of crap. Coprolites, in order to last for so long, require an arid environment. Plenty of dry caves exist in western North America, but Paisley Caves are special in that they’re the only ones known to harbor evidence of human activity dating back to the Pleistocene-Holocene transition.

That said, this evidence is not great. Aside from the supposed human coprolites, the only other evidence at Paisley Caves from this time period tends to be flakes left over from the manufacturing of stone tools (which can’t be reliably dated) and butchery marks found on the bones of possible prey animals (which might actually be gnaw marks made by non-human animals). This is where ancient poop can help—when skeletal or other lines of evidence are either scarce or non-existent.

“The most convincing evidence for many archaeologists was a collection of preserved feces containing Native American mitochondrial DNA (mtDNA) deposited 14,400 to 14,000 years ago, making them the oldest directly dated human remains in the Western Hemisphere,” John Blong, a co-author of the new study and an archaeologist at Newcastle University, wrote in an email to Gizmodo. “This evidence was criticized, though, because several of the coprolites contained both human and dog mtDNA, suggesting that the human mtDNA may be the result of contamination from overlying layers. No one doubts that the coprolites are as old as the radiocarbon dates say they are, they just doubt they are human.”

That the prior mtDNA analysis was deficient is a distinct possibility. People and animals have been sharing these caves for millennia, so it’s very possible that genetic material from one pile of poop leached onto others.

“If you’ve ever watched a crime show on TV, you know that DNA can get everywhere,” said Blong. “Organisms constantly shed DNA in hair, skin cells, sweat, saliva, and so on.” At the same time, DNA is soluble in water, making it highly mobile in wet environments.

“Even though the interior of the Paisley Caves is very dry, we do see clues in the sediments that there were occasional, short-term wetting events,” Blong explained. “Imagine a storm with heavy rain that blows into the caves. The rain soaks into the cave sediments and dries a day or two later. Even in this short period of time, the water can transport human DNA left by a later group down into the deeper sediments representing an earlier time.”

Thankfully, DNA isn’t the only clue available to scientists, as coprolites also contain fecal lipid biomarkers, which can be pinned to certain types of animals. Moreover, lipids—organic molecular compounds that include fats, oils, steroids, and other biosignatures—are not very soluble in water, so they don’t tend to move around caves when things get wet. They’re also chemically stable, so they preserve well over long timescales.

“These characteristics make lipids a more reliable source for identifying human coprolites in a setting where cave sediments occasionally get wet,” said Blong.

With this in mind, Blong, along with study co-author Lisa-Marie Shillito and other colleagues, analyzed the lipid biomarkers found in 21 coprolite samples taken from Paisley Caves, all of which were previously found to be of human origin through mtDNA analysis. The researchers ran tests to determine sterol and bile content, in order to discern human feces from those produced by other animals. The researchers then compared these samples to the surrounding sediment, finding that minimal leaching occurred between the coprolites and the cave environment.

Of the 21 samples analyzed, 13 were identified as belonging to humans, two of which had been previously dated to the 14,000-year-old timeframe. Interestingly, one poop sample was linked to a panther and another to a lynx. Details of this analysis were published in Scientific Reports.

“Our study addresses persistent criticisms of the DNA evidence for the earliest human occupation of the Paisley Caves,” said Blong. “We address issues of potential DNA contamination through fecal lipid biomarker analysis, providing evidence that there likely was DNA moving from younger human occupations into older cave sediments and coprolites, but also confirming that people were camping at the caves as early as 14,200 years ago.”

Katelyn McDonough, a Ph.D. candidate in the Department of Anthropology at Texas A&M University, told Gizmodo that the fecal biomarker approach is “very exciting,” as lipids “preserve better and move less than other materials, such as DNA.” Overall, “this study both advances and showcases the fecal biomarker approach and makes a good case for the use of this method in tandem with DNA analysis in the future,” said McDonough, who wasn’t involved in the new research, though she has spent time working in Paisley Caves.

McDonough said she was “somewhat surprised” by the disagreement between the DNA and biomarker readings for some of the coprolites, “but that goes to show that we shouldn’t always rely solely on DNA and that multiple lines of evidence are best, when possible.”

For the new study, the authors also directly dated a cultural remnant found in the caves. A bulrush fragment, either from a basket or mat, was found to be roughly 14,000 years old, “further confirming the earliest human occupation,” said Blong. McDonough said the directly dated piece of basketry is “incredible” and an “extremely unique glimpse into plant use and textile production around 14,000 years ago.”

“We still have a lot to learn about when the first people arrived in the Americas, where they came from, and what routes they took to get here,” said Blong. “Our study adds to growing evidence that people were in the Americas more than 14,000 years ago, prior to the widespread Clovis culture.”

Indeed, the new paper is further evidence that humans reached this part of the world prior to the emergence of Clovis culture and its iconic stone tool technology. The Clovis people, who emerged around 11,500 to 11,000 years ago, were once considered to be the first inhabitants of North America, but this theory is increasingly coming into doubt. Archaeological evidence excavated in western Idaho suggests humans were in the region well over.

As Blong pointed out, these coprolites are the oldest directly dated human remains in the Western Hemisphere, but there’s other important archaeological evidence to consider. A study from last year, for example, showcased some of the earliest evidence of humans in North America, specifically at the Cooper’s Ferry site in western Idaho. Stone tools, animal bones, traces of fire pits, and other signs of human occupation were dated to between 16,560 and 15,280 years ago.

It’s also worth pointing out that the colossal ice sheet separating North America from Siberia began melting around 14,800 years ago. That humans were living in Oregon’s Paisley Caves soon afterward is not much of a surprise, but it’s good to have this extra bit of poopy evidence. Excitingly, these human coprolites have more stories to tell. As Blong told Gizmodo, he and his colleagues are currently analyzing the coprolites to figure out what these pioneering humans were eating.

1,000-Year-Old Buffalo Jump Discovered in Wyoming

For Curt Ogburn and Wade Golden, the journey to the excavation of the Ogburn-Golden Bison Jump near Baggs began almost 30 years ago, when the two high school students first found an old bison skull on state land.

Wade and I were like brothers,” Ogburn said. We found all kinds of stuff out in the desert, and it was our playground. One day in 1991, Ogburn and Golden were out in the hills, doing the thing so many Wyoming teenagers do: scaling rocks, climbing hills, and exploring.

“We were hiking around the Red Desert west of Baggs, and found a buffalo skull,” Golden said. “We thought, ‘Wow, this is cool,’ so we picked it up and took it back to the high school science lab where we gathered around and looked at it… That was it.”

In about 2008, Golden started wondering — could he find that site again?

“As I was hiking in, I started finding artifacts. I found a lot more buffalo skulls and a lot more bones all around,” Golden said. Golden said that after a few attempts to contact the Wyoming State Archaeologist, Spencer Pelton, who took the job in November, agreed to investigate.

“I get a lot of calls, but this one seemed promising,” Pelton said, adding that the two first headed to the site in late June.

“We discovered this was an archaeology site used to kill bison, and it was used between around 1,000-1,500 years ago,” he said. “This particular site is — the best way to put it is that there is no way that bison bone would have ended up where it was without humans having a role in it. These bison bones are perched upon some pretty steep, cliffy areas, and it is not in a place where you would expect an animal to die naturally.”

For what were the earliest users of the bow and arrow, the journey to the area started 1,000-1,500 years ago. This was a millennium before the introduction of the horse to North America, around the time people transitioned from using a spear, or atlatl, to the bow and arrow. Several arrow points directly associated with the bones have also been discovered.

“These are probably some of the first people to use the bow and arrow,” Pelton said. “The site wasn’t so much of a cliff, but a steep embankment where the animals would become increasingly confined near steep, broken terrain. As these bison got more and more confined, it got easier for people to kill them.”

Bison hunting was a very dangerous endeavor, he said.

“To some extent, hunting is still dangerous compared to our day-to-day lives,” Pelton said. “But especially in prehistoric days, chasing these giant animals with stone tools, there is not a lot of room for error in that process.”

Landscape at any given site is unique, but the bones at the Ogburn-Golden Bison Jump were preserved in a mudstone layer of rock.

“The bones became incorporated with the sediments in this area, which have gradually eroded down to where we found them today,” Pelton said. “This place is in a unique setting. I have never actually seen anything like it.”

Slopes are typically not great for preservation because they’re constantly eroding, but in this case, the bedrock mudstone preserved the bones in just the right way, Pelton said. Wyoming likely had its prehistoric population peak around 1,000-1,500 years ago, when people lived in semi-permanent seasonal camps that they would return to on an annual basis. There is evidence that people in the area had a complex, large society with an extensive trade network reaching the Pacific Ocean.

The exact location of the Ogburn-Golden Bison Jump is confidential for the time being, to preserve the opportunity for study.

“It is really important that when we find an archaeology site of some significance that one, there is confidentiality involved,” Pelton said. “We don’t want folks collecting things because that would impact our ability to interpret how the site was used, and two, this site, in particular, doesn’t lend well to public interpretation. It is in a really dangerous spot, so how we handle something like this is we conduct an excavation, write up our findings and establish an exhibit in a local museum.”

For Golden, preservation is first and foremost.

“This is history that should be shared with everyone,” he said. “It is very neat. I knew immediately, once I started finding artifacts, what it was, and the importance of it. Ogburn said that to a 16-, 17-year-old kid, finding the bison skull was a novelty, but it is only now that he can fully appreciate what he and his best friend stumbled on three decades ago.

“We knew what it was, but we didn’t know the extent until the State Archaeologist came to look at the site,” he said. “Wade has always been the geologist, archaeologist type. We grew up together since we were four years old, and I used to tease him that he could get out of a car in the middle of a Walmart parking lot and trip over an arrowhead. He is that kind of guy. He is lucky — but he knows what he is looking for.”

Ogburn’s own son is 10 now and loves Wyoming’s history. That his name will forever be on a bison jump in Carbon County — that is pretty amazing.

“I am just so glad Wade kept up with this. My son is 10, and he loves this kind of thing,” Ogburn said. “It is pretty cool.”

The ancient giants of Nevada and the mystery of lovelock cave

For more than a century, a story has persisted about the skeletons of giants being found in Lovelock Cave in northern Nevada. For many years, human remains were put on display in museums here and elsewhere, but that changed. Most of the bones and skulls that were once considered to be historical artifacts have been returned to tribes for burial.

If oversized bones from the so-called Lovelock giants ever existed, they are no longer available to the public. But their story behind the legend persists. Slicing through the bone-dry Humboldt sink on a long dirt road, it’s hard to imagine that all of it was once underwater.

Remnants of a vast ancient lake can still be seen in the distance. For generations of first Americans, this was a lush paradise of tules, fish, and waterfowl. Humans have climbed the same narrow path up the jagged mountain for more than 4,000 years. That’s how long indigenous peoples lived in and around the Lovelock Cave.

The roof of the cave is coated with soot from countless campfires lit by ancestors of the Paiutes. According to tribal lore, a race of red-headed giants made its last stand in the cave.

Reporter George Knapp: “Among today’s Paiutes, do most say the giants were real?”

Devoy Munk: “All that I’ve talked to say yes. I’ve haven’t heard anybody say no.”

Devoy Munk, a Lovelock historian, has spent all of her 80-plus years in Lovelock. Her family’s home today houses a small museum, jam-packed with artifacts and depictions documenting centuries of native culture and pioneer life.

Munk has earned the trust of Paiute elders who say the stories are true, and that the red-headed interlopers not only killed but ate their ancestors.

“My Indian friends tell me they were cannibals, that they set traps. They dug holes in pathways where they walked, covered them, and then Indians would fall in, and they said the best parts to eat were the thighs,” Munk said.

On Internet sites and alien-themed TV shows, the gruesome legend has blossomed, but it’s hardly new. Versions have been told and retold in magazines, even scholarly journals for more than a century.

Famed Nevadan Sarah Winnemucca first wrote in her acclaimed book that the Paiutes waged a three-year war against a tribe of red-headed cannibals before trapping — then killing — the last of them inside lovelock cave.

Her book doesn’t mention giants, and mainstream archeologists have vigorously rejected the entire story, to the point that the state museum in Winnemucca admonishes visitors at its front entrance that the red-headed giants are a myth.

“There have been skeletons pulled out of the Reid collection,” said Bill Snodgrass. “They found some in there roughly 6’2″. When you think about it, back then, six-foot was a very tall individual.”

Snodgrass is the curator of the Marzen House Museum in Lovelock and thinks there is a reasonable basis for parts of the story. In the early 20th century, guano miners began excavating the Lovelock Cave and uncovered thousands of artifacts along with mummified remains including a few specimens much taller than the typical Paiute of centuries past.

Later scientific excavations found troves of native antiquities along with bones. Some human remains were destroyed. Others went to museums for display. A few, Snodgrass says, were consumed in bizarre initiation rituals. He adds there is evidence — including basketry — of an unknown culture that lived near the cave. Records show some of them had red hair.

“I can’t say who they were, redheads or not. Some say uric acid changes the color of hair, but there was definitely a different people here,” Snodgrass said.

Many, if not most, of the visitors who end up at the Marzen House Museum, have questions about the red-headed cannibals. The locals still enjoy the debate.

Reporter George Knapp: “The two of you disagree but it’s a friendly disagreement?”

“Oh yes. We’ve had this discussion and he hasn’t convinced me and I haven’t convinced him,” Munk said. Whether the red-headed giants ever existed, visits to the two museums are worth the drive.